||Investigation by using Sambiloto etract as antibacterial agent of Staphylosccocus aureus and Coagulase Negative Staphylococci should be done to solve mastitis problems. The eperiment was used 70 milk samples that collected from Nongkojajar and Batu Dairy Herda for indentification and isolation of Staphylococccus aureus and Coagulase Negative Staphylococci. From mastitic milk sanples were identified isolate S. Aureus by Staphylococci are perfectl spherical cells about 1 micrometer in diameter and grow in clusters, β hemolysis, coagulase (+), catalase (+) and Gram (+), however Coagulase Negative Staphylococci coagulase (-). Antibacteria test against some bacteria from the milk of dairy cows mastitis performed using the diffusion method of Kirby Bauer. Antibacterial discs were given the extract powder of sambiloto 0 mg, 125 mg and 500 mg 70 cc of water solvent. The result showed that there was no antibacterial activity from sambiloto etract againts isolates of Staphylococcus aureus and Coagulase Negative Staphlococci (CNS) were collected from Nongkojajar and Batu Dairy Herds.