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Judul Perbedaan Konsentrasi Bahan Pemutih Gigi terhadap Sitotoksisitas Menggunakan ESEI MTT (The Differences Concentration of Tooth Whitening Maternal Toward Cytotoxicity Using MTT Assay)
No. Panggil Jurnal Penelitian Medika Eksakta: 8(1):8-15
ISBN/ISSN 1411-6626
Pengarang Asti Meizarini - Personal Name
Judul Seri
GMD Text
Bahasa Ina
Penerbit Lemlit dan Pengabdian Unair
Tahun Terbit 2009
Tempat Terbit Surabaya
Deskripsi Fisik 8p,
Abstrak/Catatan The aim of this study was to evaluate the cytotoxicity of several tooth whitening material, carbamide peroxide 10%, 15%, 20% and hydrogen peroxide 38% toward BHK-21 cell using MTT assay. Each well of microplates which used for the test were aliquotted BHK-21 cell suspension, after that the test solution were added to eight well each group, respectively. Microplates were incubated at 37⁰C in 5% CO₂ incubator for 20 hour. MTT stock was added to each well, inclubate again for 4 hours, after that DMSO was added. The microplate were detected using ELISA reader at 630 nm. The cytotoxicity was evalauetd by the proliferation oercentages of cells treated with various concentrations of tooth whitening gel, and comparing the results with that of the positive and the negative control. The result showed showed that percentages of the living cell at 10% carbamide peroxide group = 86,73%; 15% = 81,22%; 20% = 81,82%; 38% hydrogen peroksida = 64,08%, respectively. Anova test and LSD showed no significant difference between 10%, 15%, 20% groups, but there are significant toward 38% group and controle. The 38% hydrogen peroxide group is expectable to bemore cytotoxic than those containing 10% carbamide peroxide releases oxygen. Exposure to higher concentrations of hydrogen peroxide can increased the oxidative reactions and damages by free radicals. The oxidative reactions and subsequent damage in cells are the major mechanisms responsible for the toxicity of peroxide compounds. Conclusion. The 10%, 15%, 20% carbamide peroxide and 38% hydrogen peroxide tooth whitening agents conclusion were not detected cytotoxic toward BHK-21 cell line using MT assay within CD₅₀.
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